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  • Sample Size: 250-500 mg of soil
  • Expectant Yield: up to 5 µg of genomic DNA
  • Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
  • Operation Time: within 40 minutes
  • Elution Volume: 30-100 µl
  • Kit Storage: dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 lysis Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal Buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.


Component

IB47800

IB47801

IB47802

SL1 Buffer1

4 ml

50 ml

85 ml

SL2 Buffer

1 ml

15 ml

30 ml

SL3 Buffer

10 ml

45 ml x 2

160 ml

Wash Buffer2
(Add Ethanol)

1 ml
(4 ml)

25 ml
(100 ml)

25 ml
(100 ml)

Elution Buffer

1 ml

6 ml

30 ml

Inhibitor Removal Columns

4 pcs

50 pcs

100 pcs

GD Columns

4 pcs

50 pcs

100 pcs

Beadbeating Tubes

4 pcs

50 pcs

100 pcs

2 ml Centrifuge Tubes

4 pcs

50 pcs

100 pcs

2 ml Collection Tubes

4 pcs

50 pcs

100 pcs

Item#:
ASDNARNAKIT26

PCR DNA Clean-up Kit,

4 x 96 Well Format

High Throughput PCR Clean-Up


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.


Sample Size:

Up to 50µl of PCR product
Up to 50mg of agarose slice

Format:

96-Well Plates

Operation:

Centrifuge/Vacuum manifold

Binding Capacity:

10ug per well

DNA Size:

50bp-10kb

Recovery:

80-90% for Gel Extraction
90-95% for PCR Clean-Up

Operation Time:

30 min for PCR Clean-Up
40 min for Gel Extraction


Item#:
IB47040
Your Price:
462.07
Each

PCR DNA Clean-up Kit,

10 x 96 Well Format

High Throughput PCR Clean-Up


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.


Sample Size:

Up to 50µl of PCR product
Up to 50mg of agarose slice

Format:

96-Well Plates

Operation:

Centrifuge/Vacuum manifold

Binding Capacity:

10ug per well

DNA Size:

50bp-10kb

Recovery:

80-90% for Gel Extraction
90-95% for PCR Clean-Up

Operation Time:

30 min for PCR Clean-Up
40 min for Gel Extraction


Item#:
IB47050
Your Price:
1052.90
Each

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
  • Tube volume: 250µl
  • Dialysis volume: 10-250µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 0.4cm x 1.1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASDNARNAKIT29

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
  • Tube volume: 800µl
  • Dialysis volume: 50-800µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 1cm x 0.5cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification.
Item#:
ASDNARNAKIT28

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
  • Tube volume: 3ml
  • Dialysis volume: 0.1-3ml
  • Min. sample size for extraction: 20µg
  • Max. gel slice: 2cm x 1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp


Item#:
ASDNARNAKIT27

The Total RNA MINI and MAXI kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RT-PCR, Real Time PCR and northern blotting.

 

Sample Size

MINI-up to 100 mg of fresh plant tissue/ 25mg of dry plant tissue

MAXI-up to 500 mg of fresh plant tissue

Format Spin Columns
Yield

MINI-5-30 ug for young leaf

MAXI-50-300 ug for young leaf

Binding Capacity MINI-up to 60 ug
Elution Volume MAXI-up to 500 uL
Operation Time

MINI-60 minutes or less

MAXI-60 minutes

Applications RT-PCR, Real Time  RT-PCR, Northern Blotting, Primer Extension, RNase Protection Assays, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT35

IBI's Viral Nucleic Acid Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids and the supernatant of viral-infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays.

Item#:
ASDNARNAKIT39

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

  • Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
  • Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
  • A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
  • Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

Item

Volume

Product

Shipping

Storage

IBI Isolate

4 mL

IB47630

Room Temperature

Dry at 2°C to 25°C for up to 9 months

80 mL

IB47631

160 mL

IB47632

W1 Buffer

2 mL

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

50 mL

IB47631

130 mL

IB47632

Wash Buffer*
(Add Ethanol)

2 mL (8 mL)

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

50 mL (200 mL)

IB47631

25 mL + 50 mL (50 mL + 200 mL)

IB47632

RNase-free Water

1 mL

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

6 mL

IB47631

15 mL

IB47632

RB Columns

4 pcs

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

100 pcs

IB47631

200 pcs

IB47632

2 mL Collection Tubes

8 pcs

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

200 pcs

IB47631

400 pcs

IB47632

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.

IB47630

IBI Tri-Isolate Total RNA Kit, 4 Preps

IB47631

IBI Tri-Isolate Total RNA Kit, 100 Preps

IB47632

IBI Tri-Isolate Total RNA Kit, 200 Preps

Item#:
ASDNARNAKIT38
  • Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
  • Yield: 5-30 µg
  • Format: Spin column
  • Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control 
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Kit

IB47300

IB47301

IB47302

IB47303

RB Buffer

2 mL

30 mL

60 mL

130 mL

W1 Buffer

2 mL

30 mL

50 mL

130 mL

Wash Buffer*
(Add EtOH)

1 mL
(4 mL)

12.5 mL
(50 mL)

25 mL
(100 mL)

50 mL x 2
(200 mL x 2)

RNase-free H2O

1 mL

6 mL

15 mL

30 mL

RB Column

4 pcs

50 pcs

100 pcs

300 pcs

Filter Column

4 pcs

50 pcs

100 pcs

300 pcs

2 mL Collection Tubes

8 pcs

100 pcs

200 pcs

600 pcs

Micropestle

4 pcs

50 pcs

100 pcs

300 pcs

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution 
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

Item#:
ASDNARNAKIT21

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood

Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:

Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis

Item#:
ASDNARNAKIT1

IBI Small DNA Fragment

Extraction Kit (50-200bp),

fficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
IB47062
Your Price:
390.34
Each
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